Application of Monolithic Silica Column for HPLC Separation of Estrogens
نویسندگان
چکیده
A monolithic silica column has recently been widely used for HPLC separation analysis. Compared with the particle−packed column (conventional column), the monolithic silica column eluted the analyte with a smaller retention factor (k), lower pressure and satisfactory separation [1]. However, the monolithic silica column has not been widely used for the HPLC or LC/MS analysis of steroids. These results prompted us to compare a monolithic silica column, Chromolith Performance RP−18e, with the representative conventional columns, J’sphere ODS−H80, −M80, −L80, regarding their chromatographic behavior during the separation of estrogens as model steroids (Fig. 1). The application of the monolithic silica column for the separation of products formed in vitro and the photocatalytic degradation experiments using estrogen as a substrate is also described.
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